A reliable method for quantification of splice variants using RT-qPCR
نویسندگان
چکیده
منابع مشابه
Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
MicroRNAs are a class of small non-coding RNAs that serve as important regulators of gene expression at the posttranscriptional level. They are stable in body fluids and pose great potential to serve as biomarkers. Here, we present a highly specific, sensitive and cost-effective system to quantify miRNA expression based on two-step RT-qPCR with SYBR-green detection chemistry called Two-tailed R...
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متن کاملQuantification of splice variants using real-time PCR
A reliable and robust method for measuring the expression of alternatively spliced transcripts is an important step in investigating the significance of each variant. So far, accurate quantification of splice variants has been laborious and difficult due to the intrinsic limitations of conventional methods. The many advantages of real-time PCR have made this technique attractive to study its ap...
متن کاملMethods and platforms for the quantification of splice variants' expression.
The relatively limited number of human protein encoding genes highlights the importance of the diversity generated at the level of the mRNA transcripts. As alternative RNA splicing plays a key role in mediating this diversity, it becomes critical to develop the tools and platforms that will deliver quantitative information on the specific expression levels associated with splice isoforms. This ...
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ژورنال
عنوان ژورنال: BMC Molecular Biology
سال: 2016
ISSN: 1471-2199
DOI: 10.1186/s12867-016-0060-1